試薬・器具

室温棚、4℃, -20℃, -80℃, 液体窒素, incubator, clean bench, 遠心機(14ml, 50ml), water bath (Taitec サンボックス#10), microscope, おもり(water bath中で瓶が浮かないようにする), electroporator, waterbath, 液体窒素, pipette

φ10cm dish (FALCON 3003)
φ6cm dish (FALCON 3004)
12 well plate (FALCON 3043)
24 well plate (FALCON 3047)
48 well plate (FALCON 3078)
60 well plate (Nunc 163118)
6ml tube (FALCON 2058)
14ml tube (FALCON 2057)
PFm用 50ml tube (CORNING 25331-50)
ES用 50ml tube (FALCON 2070)
Pipette 1ml (FALCON 6520), 2ml(6507), 5ml(6543), 10ml(6551), 25ml(6535), 50ml(6550)
セラムチューブ2ml (住友ベークライトMS-4503)
フィルター(sartorius Minisart SM16534 0.20μm)

PHOSPHATE BUFFERED SALINE TABLETS: SIGMA P-4417 50tablets
Water "Cell Culture Grade": IBL Code No.33617 500ml
Fetal Bovine Serum (Lot checkしたもの:GIBCO cat. #10437-028 lot 1023388)¥2.7-3万
DMEM: IBL 33253S ¥4500
Neo耐性初代培養細胞オリエンタル酵母工業（株）Cat.No.YE9284300　¥3.0万
初代培養細胞オリエンタル酵母工業（株）Cat.No.YE9284400 ¥1.5万
NEAA: Non-essential amino acids
G-418: Geneticin Sigma G5013 5g GIBCO 11811-031
→ 加藤研ではG418M: 320μg/ml (ESM 25ml + G418(100mg/ml) 80μl)
modified : G418 (net100mg/ml) 125µl + ESM 40ml (by M. I.)
p-ヒドロキシ安息香酸: p-hydroxybenzoic acid Wako 084-04102 25g RT保存 &bsl;
Trypsin-EDTA: IBL Code No. 23315
Gelatin: Sigma G-1890
Mitomycin C: Sigma M-0503
LIF(ESGRO): AMRAD AM1101
2-Mercaptoethanol: Sigma M-6250
DMSO: KANTO CHEMICAL CO.,INC. 2950-1B
Adenosine: Sigma A-9251
Guanosine: Sigma G-6752
Cytidine: Sigma C-9505
Uridine: Sigma U-3750
Thymidine: Sigma T-9250

2-mercaptoethanol, PBS,ゼラチン,水,安息香酸,DMSO,ゼラチンボトル, Trypan blue stain, 1.5ml Eppendorf, electroporating cuvette, フィルター, Pipette tips,手袋,microscope, water bath, clean bench用 pipetman, はさみ, counter, 血算盤, 電卓, タイマー, マジック,1L bottle x3, 500ml bottle x2

FCS (bottle, heat inactivated aliquot); NS(100x nucleosides), TE(trypsin-EDTA) bottle, 0.25% TE, 0.05% TE, tube rack, G418 tubes, LIF tubes

ESM tube, PBS tube, PFM tube, ESM bottle, PFM bottle, MMC eppen, NEAA bottle, G418 vial, DMEM, PBS(feeder)x 2, PBS(ES)x1