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Author: Ueda, T.; Emi, M.; Suzuki, H.; Komiya, A.; Akakura, K.; Ichikawa,
T.; Watanabe, M.; Shiraishi, T.; Masai, M.; Igarashi, T.; Ito,
H.
Year: 1999
Title: Identification of a I-cM region of common deletion on 13q14
associated with human prostate cancer
Journal: Genes Chromosomes Cancer
Volume: 24
Issue: 3
Pages: 183-90
Label: 99381004
Keywords: Adrenal Gland Neoplasms/genetics/secondary
*Chromosome Deletion
Chromosomes, Human, Pair 13/*genetics
Genes, Suppressor, Tumor
Human
Liver Neoplasms/genetics/secondary
Loss of Heterozygosity/genetics
Lymphatic Metastasis
Male
Microsatellite Repeats
Neoplasm Metastasis/genetics
Prostatic Neoplasms/*genetics
Support, Non-U.S. Gov't
Abstract: Frequent allelic losses on chromosome arm 13q are observed in
carcinomas of the head and neck, breast, ovary, and pituitary
gland. We analyzed 59 primary prostate tumors (stage B, 18 patients;
C, 12 patients; D1, 4 patients; and endocrine therapy-resistant
cancer death, 25 patients), as well as 18 metastatic tissues from
14 of the 25 cancer death patients for loss of heterozygosity
(LOH) using 35 microsatellite markers on chromosome arm 13q. Of
the 59 primary tumors, 31 (53%) showed LOH involving at least
one locus. Detailed deletion mapping identified a distinct commonly
deleted region in the I-cM interval flanked by D13S153 and D13S273
on 13q14 and this region overlapped a part of the RB1 gene. Paired
DNAs were available from both primary and metastatic tumors in
the 14 cases of cancer death; among those pairs, we detected LOH
on 13q in seven (50%) primary tumors, and in all metastatic foci
(P = 0.0029). Moreover, the regions lost in metastatic tissues
were more extensive than those seen in the corresponding primary
tumors. These results suggest that inactivation of a putative
tumor suppressor gene(s) including the RB1 gene on 13q14 plays
an important role in human prostate cancer.